产品描述
This Rat VEGFR-2/Flk-1 ELISA Kit was designed for the quantitative measurement of Rat VEGFR-2/Flk-1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.
别名
Kdr ELISA Kit; Flk1 ELISA Kit; Vascular endothelial growth factor receptor 2 ELISA Kit; VEGFR-2 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fetal liver kinase 1 ELISA Kit; FLK-1 ELISA Kit; Protein-tyrosine kinase receptor flk-1 ELISA Kit; CD antigen CD309 ELISA Kit
缩写
KDR
Uniprot No
O08775
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 103 | |
| Range % | 98-107 | |
| 1:2 | Average % | 96 | |
| Range % | 91-100 | |
| 1:4 | Average % | 90 | |
| Range % | 83-97 | |
| 1:8 | Average % | 99 | |
| Range % | 93-105 | |
回收率
| The recovery of rat VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 107 | 101-113 | |
| EDTA plasma (n=4) | 94 | 90-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.954 | 2.911 | 2.933 | 2.783 | | | 50 | 1.933 | 1.973 | 1.953 | 1.803 | | | 25 | 1.365 | 1.480 | 1.423 | 1.273 | | | 12.5 | 0.894 | 0.885 | 0.890 | 0.740 | | | 6.25 | 0.558 | 0.599 | 0.579 | 0.429 | | | 3.12 | 0.313 | 0.328 | 0.321 | 0.171 | | | 1.56 | 0.222 | 0.230 | 0.226 | 0.076 | | | 0 | 0.145 | 0.154 | 0.150 | | |
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货期
3-5 working days