产品描述

This human LOXL2 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human LOXL2 in multiple samples, including serum, plasma, cell lysates, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the LOXL2 levels in the sample and is measured at 450 nm through a microplate reader.

In physiological conditions, LOXL2 has been involved in the extracellular matrix (ECM) remodeling. Similar to the other members of the LOX family, LOXL2 induces the crosslinking of ECM collagen and elastin by the deamination of peptidyl lysine residues. Other functions of LOXL2 include the regulation of the epithelial-to-mesenchymal transition, epithelial cell polarity, and differentiation mediated by transcriptional repression mechanisms. LOXL2 has been identified as the primary enzyme promoting network formation of collagen and elastin fibers during human and experimental liver fibrosis of various etiologies as well as hepatocellular carcinoma (HCC) metastasis. LOXL2 is also involved in tumor progression and metastasis of several tumor types, including breast carcinomas.

别名

LOR 2 ELISA Kit; LOR2 ELISA Kit; LOX L2 ELISA Kit; LOXL 2 ELISA Kit; LOXL2 ELISA Kit; LOXL2_HUMAN ELISA Kit; Lysyl oxidase homolog 2 ELISA Kit; Lysyl oxidase like 2 ELISA Kit; Lysyl oxidase like protein 2 ELISA Kit; Lysyl oxidase related 2 ELISA Kit; Lysyl oxidase related protein 2 ELISA Kit; Lysyl oxidase related protein WS9 14 ELISA Kit; Lysyl oxidase-like protein 2 ELISA Kit; Lysyl oxidase-related protein 2 ELISA Kit; Lysyl oxidase-related protein WS9-14 ELISA Kit; WS9 14 ELISA Kit

缩写

LOXL2

Uniprot No

Q9Y4K0

灵敏度

9.375 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LOXL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		81-95
1:2		Average %		91
		Range %		86-97
1:4		Average %		97
		Range %		91-102
1:8		Average %		102
		Range %		97-107

回收率

The recovery of human LOXL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-99
EDTA plasma (n=4)		95		90-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2400 2.619 2.519 2.569 2.413 1200 1.890 1.790 1.840 1.684 600 1.027 1.017 1.022 0.866 300 0.512 0.502 0.507 0.351 150 0.319 0.309 0.314 0.158 75 0.246 0.236 0.241 0.085 37.5 0.199 0.197 0.198 0.042 0 0.156 0.155 0.156

货期

3-5 working days