产品描述
This mouse NGAL ELISA kit is suitable for the quantitative determination of mouse NGAL in the serum, plasma, tissue homogenates, or urine. It employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with NGAL. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated antibody preparation specific for NGAL. The competitive inhibition reaction is launched between pre-coated NGAL and NGAL in samples. A substrate solution is added to the wells and the color develops oppositely to the amount of NGAL in the samples. The color development is stopped and the intensity of the color is measured.
NGAL, also called LCN2, is released by neutrophils at sites of infection and inflammation to participate in the antibacterial defense through iron sequestration. NGAL is also responsible for iron delivery to the cytoplasm where it is accumulated and activates or inhibits iron-responsive genes. Iron unloading depends on the cycling of NGAL through acidic endosomes. Devireddy LR et al have shown that NGAL is also involved in apoptosis-dependent deprivation of trophic factors. NGAL is considered the biochemical gold standard for the early diagnosis of acute kidney injury because it is rapidly released after tubular damage.
别名
Lcn2 ELISA Kit; Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL ELISA Kit; Lipocalin-2 ELISA Kit; Oncogene 24p3 ELISA Kit; 24p3 ELISA Kit; SV-40-induced 24p3 protein ELISA Kit; Siderocalin LCN2 ELISA Kit; p25 ELISA Kit
缩写
LCN2
Uniprot No
P11672
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:200 | Average % | 105 | | |
| Range % | 101-108 | | |
| 1:400 | Average % | 97 | | |
| Range % | 95-99 | | |
| 1:800 | Average % | 95 | | |
| Range % | 90-99 | | |
| 1:1600 | Average % | 90 | | |
| Range % | 84-96 | | |
回收率
| The recovery of mouse NGALspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 90 | 87-93 | | |
| EDTA plasma (n=4) | 97 | 92-102 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | pg/ml | OD1 | OD2 | Average | | | | | 800 | 0.135 | 0.141 | 0.138 | | | | | 400 | 0.258 | 0.265 | 0.262 | | | | | 200 | 0.501 | 0.525 | 0.513 | | | | | 100 | 0.955 | 0.985 | 0.970 | | | | | 50 | 1.545 | 1.574 | 1.560 | | | | | 25 | 2.088 | 2.122 | 2.105 | | | | | 12.5 | 2.457 | 2.588 | 2.523 | | | | | 0 | 2.863 | 2.698 | 2.781 | | | |
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
