产品描述

This human IL1A ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL1A in multiple samples, including serum, plasma, cell culture supernates, tissue homogenates, or cell lysates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of IL1A bound in the initial step.

IL1A is constitutively expressed in epithelial, endothelial, and stromal cells and can be upregulated in hematopoietic and non-hematopoietic cells by diverse stimuli, including TLR agonists, inflammatory cytokines, and oxidative stress. In non-hematopoietic cells, IL1A signals via IL1R on myeloid cells in a MyD88/RIPK1/(TAK1)-dependent manner to induce the expression of pro-inflammatory cytokines. IL1A also plays a role in neutrophil recruitment and host defense after infections caused by viral, fungal, or protozoan. Dysregulation of IL1A production is associated with numerous auto-inflammatory disorders.

别名

BAF ELISA Kit; FAF ELISA Kit; Hematopoietin 1 ELISA Kit; Hematopoietin-1 ELISA Kit; IL 1 alpha ELISA Kit; IL 1A ELISA Kit; IL-1 alpha ELISA Kit; Il-1a ELISA Kit; IL1 ALPHA ELISA Kit; IL1 ELISA Kit; IL1A ELISA Kit; IL1A_HUMAN ELISA Kit; IL1F1 ELISA Kit; Interleukin 1 alpha ELISA Kit; Interleukin-1 alpha ELISA Kit; Interleukin1 alpha ELISA Kit; LAF ELISA Kit; LEM ELISA Kit; Preinterleukin 1 alpha ELISA Kit; Pro interleukin 1 alpha ELISA Kit

缩写

IL1A

Uniprot No

P01583

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

85

Range %

80-92

1:2

Average %

87

Range %

81-92

1:4

Average %

90

Range %

84-96

1:8

Average %

93

Range %

85-100

回收率

The recovery of human IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type			Average % Recovery				Range
Serum (n=5)			95				90-100
EDTA plasma (n=4)			93				87-101

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 1.786 1.735 1.761 1.589 1000 1.109 1.115 1.112 0.940 500 0.627 0.678 0.653 0.481 250 0.386 0.413 0.400 0.228 125 0.292 0.301 0.297 0.125 62.5 0.238 0.257 0.248 0.076 31.25 0.223 0.213 0.218 0.046 0 0.175 0.169 0.172

货期

3-5 working days