产品描述

This Human PADI4 ELISA Kit was designed for the quantitative measurement of Human PADI4 protein in serum, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

EC 3.5.3.15 ELISA Kit; HL 60 PAD ELISA Kit; HL-60 PAD ELISA Kit; PAD 4 ELISA Kit; PAD ELISA Kit; PADI 4 ELISA Kit; PADI 5 ELISA Kit; PADI H protein ELISA Kit; Padi4 ELISA Kit; PADI4_HUMAN ELISA Kit; PADI5 ELISA Kit; PDI 4 ELISA Kit; PDI 5 ELISA Kit; PDI4 ELISA Kit; PDI5 ELISA Kit; Peptidyl arginine deiminase type IV ELISA Kit; Peptidyl arginine deiminase type V ELISA Kit; Peptidylarginine deiminase IV ELISA Kit; Protein arginine deiminase ELISA Kit; Protein arginine deiminase type 4 ELISA Kit; Protein arginine deiminase type IV ELISA Kit; Protein-arginine deiminase type IV ELISA Kit; Protein-arginine deiminase type-4 ELISA Kit

缩写

PADI4

Uniprot No

Q9UM07

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PADI4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		87
		Range %		80-92
1:200		Average %		99
		Range %		91-105
1:400		Average %		99
		Range %		92-110
1:800		Average %		94
		Range %		86-98

回收率

The recovery of human PADI4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.719 2.678 2.699 2.609 5 2.223 2.189 2.206 2.116 2.5 1.427 1.402 1.415 1.325 1.25 0.782 0.763 0.773 0.683 0.625 0.407 0.379 0.393 0.303 0.312 0.249 0.239 0.244 0.154 0.156 0.158 0.148 0.153 0.063 0 0.093 0.087 0.090

货期

3-5 working days