产品描述
This Pig IL4 ELISA Kit was designed for the quantitative measurement of Pig IL4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .
别名
IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写
IL4
Uniprot No
Q04745
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-91 | |
| 1:2 | Average % | 90 | |
| Range % | 85-95 | |
| 1:4 | Average % | 89 | |
| Range % | 84-93 | |
| 1:8 | Average % | 96 | |
| Range % | 92-101 | |
回收率
| The recovery of pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 100 | 95-105 | |
| EDTA plasma (n=4) | 92 | 86-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 400 | 2.719 | 2.652 | 2.686 | 2.492 | | | 200 | 1.962 | 1.861 | 1.912 | 1.718 | | | 100 | 1.213 | 1.204 | 1.209 | 1.015 | | | 50 | 0.748 | 0.689 | 0.719 | 0.525 | | | 25 | 0.551 | 0.527 | 0.539 | 0.345 | | | 12.5 | 0.355 | 0.374 | 0.365 | 0.171 | | | 6.25 | 0.295 | 0.272 | 0.284 | 0.090 | | | 0 | 0.192 | 0.196 | 0.194 | | |
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货期
3-5 working days