产品描述
This Dog IL1B ELISA Kit was designed for the quantitative measurement of Dog IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
别名
IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写
IL1B
Uniprot No
Q28292
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 83-100 | |
| 1:2 | Average % | 94 | |
| Range % | 91-99 | |
| 1:4 | Average % | 95 | |
| Range % | 90-105 | |
| 1:8 | Average % | 94 | |
| Range % | 86-100 | |
回收率
| The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-102 | |
| EDTA plasma (n=4) | 97 | 90-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.585 | 2.675 | 2.630 | 2.512 | | | 250 | 2.319 | 2.412 | 2.366 | 2.248 | | | 125 | 1.957 | 1.972 | 1.965 | 1.847 | | | 62.5 | 1.432 | 1.453 | 1.443 | 1.325 | | | 31.25 | 0.744 | 0.719 | 0.732 | 0.614 | | | 15.63 | 0.517 | 0.508 | 0.513 | 0.395 | | | 7.8 | 0.385 | 0.377 | 0.381 | 0.263 | | | 0 | 0.118 | 0.117 | 0.118 | | |
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货期
3-5 working days