产品描述

The human IL-23 ELISA Kit is engineered for accurate measurement of human IL-23 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the IL-23 content in the sample. The color intensity is positively correlated with IL-23 content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.

IL-23 is a heterodimeric cytokine consisting of an IL-12p40 subunit and an IL-23-specific p19 subunit. It is secreted by activated dendritic cells and macrophages and can specifically amplify and stabilize the proliferation of IL-17-secreting Th17 cells. The IL-23/Th17 pathway has been involved in the development of many autoimmune diseases, such as rheumatoid arthritis (RA), systemic lupus erythematosus, and psoriatic arthritis (PsA). IL-23 signals through the IL-23R in complex with IL-12Rβ1, inducing the generation of secreted mediators such as IL-17 and IL-22, in pro-inflammatory, IL-17-secreting TCRγδ (Tγδ17), and in Th17 cells. These pro-inflammatory mediators facilitate the rapid recruitment and activation of granulocytes and macrophages that eventually result in the development of clinical symptoms.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 400 2.608 2.507 2.558 2.415 200 1.865 1.764 1.815 1.672 100 1.207 1.186 1.197 1.054 50 0.801 0.790 0.796 0.653 25 0.437 0.448 0.443 0.300 12.5 0.301 0.312 0.307 0.164 6.25 0.228 0.234 0.231 0.088 0 0.142 0.143 0.143

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