产品描述
This Horse IL-6 ELISA Kit was designed for the quantitative measurement of Horse IL-6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .
别名
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写
IL-6
Uniprot No
Q95181
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of horse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 84-98 | |
| 1:2 | Average % | 90 | |
| Range % | 85-95 | |
| 1:4 | Average % | 96 | |
| Range % | 92-101 | |
| 1:8 | Average % | 104 | |
| Range % | 96-108 | |
回收率
| The recovery of horse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 88-97 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.267 | 2.235 | 2.251 | 2.136 | | | 100 | 1.482 | 1.589 | 1.536 | 1.421 | | | 50 | 0.954 | 0.999 | 0.977 | 0.862 | | | 25 | 0.602 | 0.612 | 0.607 | 0.492 | | | 12.5 | 0.432 | 0.452 | 0.442 | 0.327 | | | 6.25 | 0.269 | 0.289 | 0.279 | 0.164 | | | 3.12 | 0.218 | 0.230 | 0.224 | 0.109 | | | 0 | 0.111 | 0.119 | 0.115 | | |
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货期
3-5 working days