产品描述
This Goat IL4 ELISA Kit was designed for the quantitative measurement of Goat IL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
缩写
IL4
Uniprot No
P79155
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 86-95 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 97 | |
| Range % | 91-103 | |
回收率
| The recovery of goat IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 96 | 90-100 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.775 | 2.799 | 2.787 | 2.645 | | | 500 | 2.109 | 1.998 | 2.054 | 1.912 | | | 250 | 1.371 | 1.387 | 1.379 | 1.237 | | | 125 | 0.791 | 0.786 | 0.789 | 0.647 | | | 62.5 | 0.505 | 0.541 | 0.523 | 0.381 | | | 31.2 | 0.357 | 0.368 | 0.363 | 0.221 | | | 15.6 | 0.278 | 0.298 | 0.288 | 0.146 | | | 0 | 0.141 | 0.143 | 0.142 | | |
|
货期
3-5 working days