产品描述
This Mouse IL-35 ELISA Kit was designed for the quantitative measurement of Mouse IL-35 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
缩写
IL-35
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 87-97 | |
| 1:2 | Average % | 95 | |
| Range % | 89-99 | |
| 1:4 | Average % | 94 | |
| Range % | 90-100 | |
| 1:8 | Average % | 93 | |
| Range % | 87-97 | |
回收率
| The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 87-98 | |
| EDTA plasma (n=4) | 96 | 91-101 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.461 | 2.391 | 2.426 | 2.235 | | | 500 | 2.073 | 1.965 | 2.019 | 1.828 | | | 250 | 1.669 | 1.577 | 1.623 | 1.432 | | | 125 | 1.272 | 1.183 | 1.228 | 1.037 | | | 62.5 | 0.814 | 0.804 | 0.809 | 0.618 | | | 31.2 | 0.567 | 0.525 | 0.546 | 0.355 | | | 15.6 | 0.335 | 0.314 | 0.325 | 0.134 | | | 0 | 0.194 | 0.188 | 0.191 | | |
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货期
3-5 working days