产品描述
This Human ITIH3 ELISA Kit was designed for the quantitative measurement of Human ITIH3 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78.13 ng/mL-5000 ng/mL and the sensitivity is 19.53 pg/mL .
别名
ITIH3 ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit; ITI heavy chain H3 ELISA Kit; ITI-HC3 ELISA Kit; Inter-alpha-inhibitor heavy chain 3 ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
缩写
ITIH3
Uniprot No
Q06033
灵敏度
19.53 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human TIH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:20 | Average % | 92 | |
| Range % | 86-99 | |
| 1:40 | Average % | 97 | |
| Range % | 92-103 | |
| 1:80 | Average % | 86 | |
| Range % | 80-89 | |
| 1:160 | Average % | 90 | |
| Range % | 83-99 | |
回收率
| The recovery of human TIH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-95 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | | | | 5000 | 0.083 | 0.085 | 0.084 | | | | 2500 | 0.139 | 0.147 | 0.143 | | | | 1250 | 0.223 | 0.220 | 0.222 | | | | 625 | 0.329 | 0.347 | 0.338 | | | | 312.5 | 0.572 | 0.614 | 0.593 | | | | 156.3 | 0.814 | 0.849 | 0.832 | | | | 78.13 | 1.228 | 1.259 | 1.244 | | | | 0 | 2.081 | 2.226 | 2.154 | | |
|
货期
3-5 working days