产品描述

This Human IL31RA ELISA Kit was designed for the quantitative measurement of Human IL31RA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

别名

Class I cytokine receptor ELISA Kit; CRL 3 ELISA Kit; CRL ELISA Kit; CRL3 ELISA Kit; Cytokine receptor like 3 ELISA Kit; Cytokine receptor-like 3 ELISA Kit; GLM R ELISA Kit; GLM-R ELISA Kit; GLMR ELISA Kit; Gp130 like monocyte receptor ELISA Kit; GP130 like receptor ELISA Kit; Gp130-like monocyte receptor ELISA Kit; Gp130-like receptor ELISA Kit; GPL ELISA Kit; HGLM R ELISA Kit; HGLM-R ELISA Kit; HGLMR ELISA Kit; IL 31 receptor subunit alpha ELISA Kit; IL 31R subunit alpha ELISA Kit; IL 31RA ELISA Kit; IL-31 receptor subunit alpha ELISA Kit; IL-31R subunit alpha ELISA Kit; IL-31R-alpha ELISA Kit; IL-31RA ELISA Kit; IL31 receptor subunit alpha ELISA Kit; IL31R subunit alpha ELISA Kit; IL31R_HUMAN ELISA Kit; IL31RA ELISA Kit; Interleukin 31 receptor A ELISA Kit; interleukin 31 receptor subunit alpha ELISA Kit; Interleukin-31 receptor subunit alpha ELISA Kit; MGC125346 ELISA Kit; PLCA 2 ELISA Kit; PLCA2 ELISA Kit; PRO21384 ELISA Kit; Soluble type I cytokine receptor CRL3 ELISA Kit; ZcytoR17 ELISA Kit

缩写

IL31RA

Uniprot No

Q8NI17

灵敏度

1.56 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL31RA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		95
		Range %		89-99
1:10		Average %		102
		Range %		96-107
1:20		Average %		96
		Range %		92-103
1:40		Average %		89
		Range %		82-94

回收率

The recovery of human IL31RA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		86		82-91
EDTA plasma (n=4)		97		92-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 400 2.943 2.875 2.909 2.819 200 2.387 2.246 2.317 2.227 100 1.554 1.512 1.533 1.443 50 0.826 0.808 0.817 0.727 25 0.467 0.455 0.461 0.371 12.5 0.259 0.246 0.253 0.163 6.25 0.179 0.173 0.176 0.086 0 0.091 0.089 0.090

货期

3-5 working days