产品描述
This Rat IL3 ELISA Kit was designed for the quantitative measurement of Rat IL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
Il3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
缩写
IL3
Uniprot No
P04823
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-98 | |
| 1:2 | Average % | 100 | |
| Range % | 95-113 | |
| 1:4 | Average % | 91 | |
| Range % | 85-97 | |
| 1:8 | Average % | 97 | |
| Range % | 92-104 | |
回收率
| The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-94 | |
| EDTA plasma (n=4) | 93 | 87-98 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.436 | 2.276 | 2.356 | 2.214 | | | 100 | 1.975 | 2.014 | 1.995 | 1.853 | | | 50 | 1.435 | 1.372 | 1.404 | 1.262 | | | 25 | 0.868 | 0.913 | 0.891 | 0.749 | | | 12.5 | 0.553 | 0.519 | 0.536 | 0.394 | | | 6.25 | 0.387 | 0.407 | 0.397 | 0.255 | | | 3.12 | 0.285 | 0.276 | 0.281 | 0.139 | | | 0 | 0.144 | 0.140 | 0.142 | | |
|
货期
3-5 working days