产品描述

The human milk fat globule-EGF factor 8 protein (MFGE8) Elisa kit is suitable for the quantitative measurement of human MFGE8 in serum, cell culture supernates, urine, or cerebrospinal fluid (CSF). This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of MFGE8 in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

MFGE8 is a multifunctional glycoprotein that is found in lacteal glands and milk fat globules and contributes to connecting αvβ3 integrin on phagocytic macrophages with phosphatidylserine on apoptotic cells thus enhancing engulfment of apoptotic cells. This process is essential for maintaining the host immune system under physiological conditions. In addition, MFGE8 also directly regulates various cellular functions, such as attenuating inflammation and healing of injured tissues. MFGE8 has been demonstrated to have anti-inflammatory and regenerating roles during intestinal inflammation. Studies have recently identified MFGE8 as a novel and outstanding modulator for vascular aging via targeting endothelial cells (ECs) and vascular smooth muscle cells (VSMCs).

别名

BA46 ELISA Kit; Breast epithelial antigen BA46 ELISA Kit; EDIL1 ELISA Kit; HMFG ELISA Kit; hP47 ELISA Kit; HsT19888 ELISA Kit; Lactadherin ELISA Kit; Medin ELISA Kit; MFG-E8 ELISA Kit; MFGE8 ELISA Kit; MFGM ELISA Kit; MFGM_HUMAN ELISA Kit; Milk fat globule EGF factor 8 ELISA Kit; Milk fat globule EGF factor 8 protein ELISA Kit; Milk fat globule-EGF factor 8 ELISA Kit; O acetyl disialoganglioside synthase ELISA Kit; OAcGD3S ELISA Kit; SED1 ELISA Kit; SPAG10 ELISA Kit; Sperm associated antigen 10 ELISA Kit; Sperm surface protein hP47 ELISA Kit

缩写

MFGE8

Uniprot No

Q08431

灵敏度

195.31 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MFGE8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		85-100
1:2		Average %		97
		Range %		92-105
1:4		Average %		91
		Range %		85-96
1:8		Average %		85
		Range %		80-93

回收率

The recovery of human MFGE8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		85-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 50000 2.437 2.452 2.445 2.264 25000 1.885 1.876 1.881 1.700 12500 1.304 1.324 1.314 1.133 6250 0.821 0.854 0.838 0.657 3125 0.595 0.584 0.590 0.409 1562.5 0.414 0.436 0.425 0.244 781.25 0.289 0.279 0.284 0.103 0 0.174 0.187 0.181

货期

3-5 working days