产品描述

This Human NR4A2 ELISA Kit was designed for the quantitative measurement of Human NR4A2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

HZF 3 ELISA Kit; HZF3 ELISA Kit; Immediate-early response protein NOT ELISA Kit; Intermediate early receptor protein ELISA Kit; NGFI B/nur77 beta type transcription factor homolog ELISA Kit; NOT ELISA Kit; Nr4a2 ELISA Kit; NR4A2_HUMAN ELISA Kit; nuclear receptor of T cells ELISA Kit; nuclear receptor related 1 ELISA Kit; Nuclear receptor subfamily 4 group A member 2 ELISA Kit; Nur related protein 1 homolog ELISA Kit; nur related protein-1; human homolog of ELISA Kit; Nurr 1 ELISA Kit; Orphan nuclear receptor NR4A2 ELISA Kit; Orphan nuclear receptor NURR1 ELISA Kit; RNR 1 ELISA Kit; RNR1 ELISA Kit; T cell nuclear receptor NOT ELISA Kit; T-cell nuclear receptor NOT ELISA Kit; TINUR ELISA Kit; Transcriptionally inducible nuclear receptor ELISA Kit; Transcriptionally inducible nuclear receptor related ELISA Kit; Transcriptionally inducible nuclear receptor related 1 ELISA Kit; Transcriptionally-inducible nuclear receptor ELISA Kit

缩写

NR4A2

Uniprot No

P43354

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NR4A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		89-101
1:2		Average %		95
		Range %		86-106
1:4		Average %		96
		Range %		90-103
1:8		Average %		95
		Range %		82-102

回收率

The recovery of human NR4A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		86-96
EDTA plasma (n=4)		93		86-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 2000 2.084 2.098 2.091 1.988 1000 1.767 1.786 1.777 1.674 500 1.359 1.322 1.341 1.238 250 0.817 0.828 0.823 0.720 125 0.521 0.534 0.528 0.425 62.5 0.340 0.332 0.336 0.233 31.25 0.285 0.274 0.280 0.177 0 0.104 0.101 0.103

货期

3-5 working days