产品描述

This Human LAMA3 ELISA Kit was designed for the quantitative measurement of Human LAMA3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.4 ng/mL-600 ng/mL and the sensitivity is 2.35 ng/mL.

别名

E170 ELISA Kit; Epiligrin 170 kDa subunit ELISA Kit; Epiligrin subunit alpha ELISA Kit; Kalinin B1 chain ELISA Kit; Kalinin subunit alpha ELISA Kit; LAMA3 ELISA Kit; LAMA3_HUMAN ELISA Kit; LAMB3 ELISA Kit; LAMC2 ELISA Kit; Laminin gamma 2 ELISA Kit; Laminin 5 beta 3 ELISA Kit; Laminin 5 gamma 2 subunit ELISA Kit; Laminin beta 3 ELISA Kit; Laminin subunit alpha-3 ELISA Kit; Laminin subunit beta 3 ELISA Kit; Laminin subunit gamma 2 ELISA Kit; Laminin-5 subunit alpha ELISA Kit; Laminin-6 subunit alpha ELISA Kit; Laminin-7 subunit alpha ELISA Kit; Nicein subunit alpha ELISA Kit

缩写

LAMA3

Uniprot No

Q16787

灵敏度

2.35 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LAMA3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		97
		Range %		89-101
1:10		Average %		106
		Range %		100-110
1:20		Average %		93
		Range %		86-96
1:40		Average %		107
		Range %		92-111

回收率

The recovery of human LAMA3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		102		95-107
EDTA plasma (n=4)		104		98-108

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 600 2.492 2.566 2.529 2.355 300 1.743 1.708 1.726 1.552 150 1.169 1.124 1.147 0.973 75 0.762 0.745 0.754 0.580 37.5 0.440 0.467 0.454 0.280 18.75 0.323 0.333 0.328 0.154 9.4 0.244 0.251 0.248 0.074 0 0.172 0.175 0.174

货期

3-5 working days