产品描述

This human KISS1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human KISS1 in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of KISS1 bound in the initial step.

KISS1 is quickly processed in serum into smaller but biologically active kisspeptins (KPs), which mainly regulate reproductive functions through its receptor KISS1R in mammals. KISS1/KPs/KISS1R signaling is involved in the onset of puberty and control of the hypothalamic-pituitary-gonadal axis. KPs play a role in numerous biological activities, including oocyte maturation, ovulation, spermatogenesis, testicular steroidogenesis, pregnancy, as well as placental migration and invasion. In many human tumors, KISS1 acts as a metastasis-inhibitor gene and KISS1/KISS1R signaling has anti-metastatic and tumor-suppressive roles in various cancers, including ovary, bladder, and colorectal cancers.

别名

KISS 1 ELISA Kit; KiSS 1 metastasis suppressor ELISA Kit; Kiss1 ELISA Kit; KISS1_HUMAN ELISA Kit; Kisspeptin 1 ELISA Kit; Kisspeptin 10 ELISA Kit; Kisspeptin 13 ELISA Kit; Kisspeptin 14 ELISA Kit; Kisspeptin ELISA Kit; Kisspeptin-1 ELISA Kit; Kisspeptin-10 ELISA Kit; Kisspeptin-54 ELISA Kit; Kisspeptin1 ELISA Kit; Malignant melanoma metastasis suppressor ELISA Kit; Metastasis suppressor KiSS 1 ELISA Kit; Metastasis suppressor KiSS1 ELISA Kit; Metastin ELISA Kit; MGC39258 ELISA Kit

缩写

KISS1

Uniprot No

Q15726

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KISS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		86-94
1:2		Average %		101
		Range %		97-103
1:4		Average %		90
		Range %		85-96
1:8		Average %		95
		Range %		91-100

回收率

The recovery of human KISS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		86		80-93
EDTA plasma (n=4)		96		92-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.842 2.963 2.903 2.775 10 2.187 2.203 2.195 2.067 5 1.148 1.226 1.187 1.059 2.5 0.755 0.743 0.749 0.621 1.25 0.349 0.365 0.357 0.229 0.625 0.222 0.205 0.214 0.086 0.312 0.202 0.200 0.201 0.073 0 0.132 0.124 0.128

货期

3-5 working days