产品描述

This human SCF ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human SCF in multiple samples, including serum, plasma, or cell culture supernates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the SCF levels in the sample and is measured at 450 nm through a microplate reader.

SCF, also called KITLG or MGF, is of neural and smooth muscle origin and is involved in the regulation of cell proliferation, differentiation, and migration. It is a cytokine that modulates hematopoiesis and other biological processes like mast cell maturation and activation. SCF-c-Kit signaling plays a key role in the maintenance of the hematopoietic stem cells (HSCs) and progenitor cells in the bone marrow. SCF is also critical in regulating the proliferation, differentiation and function of the interstitial cells of Cajal (ICCs), which are closely related to smooth muscle dysfunction.

别名

C kit ligand ELISA Kit; C-kit ligand ELISA Kit; Ckit ligand ELISA Kit; DCUA ELISA Kit; DFNA69 ELISA Kit; DKFZp686F2250 ELISA Kit; familial progressive hyperpigmentation 2 ELISA Kit; FPH2 ELISA Kit; FPHH ELISA Kit; KIT ligand ELISA Kit; Kitl ELISA Kit; KITLG ELISA Kit; KL 1 ELISA Kit; KL1 ELISA Kit; Mast cell growth factor ELISA Kit; MGF ELISA Kit; MGF stem cell factor ELISA Kit; SCF ELISA Kit; SCF_HUMAN ELISA Kit; SF ELISA Kit; SHEP7 ELISA Kit; sKITLG ELISA Kit; Soluble KIT ligand ELISA Kit; Steel factor ELISA Kit; steel, mouse, homolog of ELISA Kit; Stem cell factor ELISA Kit; Stem cell factor precursor ELISA Kit

缩写

KITLG

Uniprot No

P21583

灵敏度

0.11 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-96
1:2		Average %		86
		Range %		83-89
1:4		Average %		92
		Range %		88-97
1:8		Average %		91
		Range %		87-94

回收率

The recovery of human SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		98-107
EDTA plasma (n=4)		86		82-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 30 2.624 2.522 2.573 2.461 15 1.796 1.897 1.847 1.735 7.5 1.281 1.188 1.235 1.123 3.75 0.739 0.713 0.726 0.614 1.8 0.429 0.404 0.417 0.305 0.9 0.277 0.295 0.286 0.174 0.45 0.215 0.212 0.214 0.102 0 0.111 0.113 0.112

货期

3-5 working days