产品描述
IL18(Interleukin-18),是一种由单核细胞、巨噬细胞等多种细胞分泌的细胞因子,能够促进细胞介导的免疫反应,同时也能促进炎症反应。IL18能够刺激NK细胞产生IFN-γ、刺激Th1细胞分化等,参与了许多疾病的发生发展。人体内IL18的含量与多种炎症性疾病、肿瘤等的发生密切相关。
华美生物所提供的Mouse Interleukin 18,IL-18 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的IL18,其灵敏度为0.39 pg/mL,检测范围为1.56 pg/mL-100 pg/mL。
别名
Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写
IL18
Uniprot No
P70380
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
82-94 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-99 |
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| 1:8 |
Average % |
94 |
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| Range % |
90-100 |
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回收率
| The recovery of mouse IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
99 |
94-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
2.646 |
2.552 |
2.599 |
2.484 |
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| 50 |
2.218 |
2.186 |
2.202 |
2.087 |
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| 25 |
1.874 |
1.804 |
1.839 |
1.724 |
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| 12.5 |
1.332 |
1.329 |
1.331 |
1.216 |
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| 6.25 |
0.925 |
0.901 |
0.913 |
0.798 |
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| 3.12 |
0.527 |
0.499 |
0.513 |
0.398 |
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| 1.56 |
0.261 |
0.278 |
0.270 |
0.155 |
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| 0 |
0.119 |
0.111 |
0.115 |
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货期
3-5 working days