产品描述
CUSABIO's IFABP rat ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IFABP in serum, plasma, urine, or tissue homogenates. This assay uses an antibody specific for rat IFABP coated on a 96-well plate. IFABP present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated IFABP antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of IFABP bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.
FABP2 is the encoding gene for IFABP, which is mainly expressed in intestinal epithelial cells. IFABP is released into the circulation after small intestinal mucosal damage and its plasma levels have been linked to small intestinal diseases such as necrotizing enterocolitis and celiac disease.IFABP plays important roles in several steps of fat absorption and transport, including the uptake and trafficking of saturated and unsaturated long-chain fatty acids, targeting free fatty acids toward different metabolic pathways, protecting the cytosol from the cytotoxic effects of free fatty acids, and regulating the enzyme activity involved in lipid metabolism.
别名
Fabp2 ELISA kit; FabpiFatty acid-binding protein ELISA kit; intestinal ELISA kit; Fatty acid-binding protein 2 ELISA kit; Intestinal-type fatty acid-binding protein ELISA kit; I-FABP ELISA kit
缩写
FABP2
Uniprot No
P02693
灵敏度
0.312 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 89-101 | |
| 1:2 | Average % | 93 | |
| Range % | 87-99 | |
| 1:4 | Average % | 90 | |
| Range % | 85-95 | |
| 1:8 | Average % | 104 | |
| Range % | 98-108 | |
回收率
| The recovery of rat iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-102 | |
| EDTA plasma (n=4) | 93 | 89-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 80 | 2.695 | 2.671 | 2.683 | 2.536 | | | 40 | 1.915 | 1.901 | 1.908 | 1.761 | | | 20 | 1.306 | 1.334 | 1.320 | 1.173 | | | 10 | 0.884 | 0.899 | 0.892 | 0.745 | | | 5 | 0.517 | 0.537 | 0.527 | 0.380 | | | 2.5 | 0.375 | 0.384 | 0.380 | 0.233 | | | 1.25 | 0.211 | 0.233 | 0.222 | 0.075 | | | 0 | 0.145 | 0.149 | 0.147 | | |
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货期
3-5 working days
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