产品描述
This Goat HSPA2 ELISA Kit was designed for the quantitative measurement of Goat HSPA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.44 ng/mL-1500 ng/mL and the sensitivity is 5.86 ng/mL.
别名
HSPA2 ELISA Kit; HSP70-3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
缩写
HSPA2
Uniprot No
Q9TUG3
灵敏度
5.86 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 90-99 | |
| 1:2 | Average % | 92 | |
| Range % | 85-99 | |
| 1:4 | Average % | 95 | |
| Range % | 92-103 | |
| 1:8 | Average % | 89 | |
| Range % | 82-98 | |
回收率
| The recovery of goat HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-98 | |
| EDTA plasma (n=4) | 95 | 89-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.308 | 2.463 | 2.386 | 2.234 | | | 750 | 1.606 | 1.666 | 1.636 | 1.484 | | | 375 | 1.078 | 1.064 | 1.071 | 0.919 | | | 187.5 | 0.732 | 0.721 | 0.727 | 0.575 | | | 93.75 | 0.428 | 0.456 | 0.442 | 0.290 | | | 46.88 | 0.313 | 0.330 | 0.322 | 0.170 | | | 23.44 | 0.219 | 0.235 | 0.227 | 0.075 | | | 0 | 0.151 | 0.153 | 0.152 | | |
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货期
3-5 working days