产品描述

CUSABIO's Endothelin-1 (ET-1) mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse ET-1 in serum, plasma, or cell culture supernates. This assay uses an antibody specific for mouse ET-1 coated on a 96-well plate. ET-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated ET-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of ET-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

ET-1, also called EDN1, is a powerful endogenous vasoconstrictor and is mainly secreted by endothelial cells. In addition to the vasoconstrictive action, ET-1 also leads to fibrosis of the vascular cells and induces the generation of reactive oxygen species (ROS), especially superoxide anions, thus resulting in the development of oxidative stress. ET-1 also exerts a pro-inflammatory effect. It increases the expression of adhesion molecules on vascular endothelial cells and stimulates the aggregation of polymorphonuclear neutrophils contributing to inflammation and endothelial dysfunction.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.729 2.612 2.671 2.524 500 1.938 1.876 1.907 1.760 250 1.228 1.204 1.216 1.069 125 0.816 0.759 0.788 0.641 62.5 0.435 0.407 0.421 0.274 31.2 0.299 0.275 0.287 0.140 15.6 0.241 0.223 0.232 0.085 0 0.151 0.143 0.147

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