产品描述
This Human GSR ELISA Kit was designed for the quantitative measurement of Human GSR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 mU/mL-40 mU/mL and the sensitivity is 0.156 mU/mL.
别名
GLR1 ELISA Kit; GLUR ELISA Kit; Glutathione reductase ELISA Kit; Glutathione reductase mitochondrial ELISA Kit; Glutathione reductase; mitochondrial ELISA Kit; GR ELISA Kit; Gr1 ELISA Kit; GRase ELISA Kit; GRD 1 ELISA Kit; GRD1 ELISA Kit; GSHR_HUMAN ELISA Kit; GSR ELISA Kit; MGC78522 ELISA Kit
缩写
GSR
Uniprot No
P00390
灵敏度
0.156 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human GR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 88-95 | |
| 1:2 | Average % | 103 | |
| Range % | 96-107 | |
| 1:4 | Average % | 92 | |
| Range % | 85-95 | |
| 1:8 | Average % | 98 | |
| Range % | 92-103 | |
回收率
| The recovery of human GR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 107 | 101-112 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| mU/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.827 | 2.787 | 2.807 | 2.648 | | | 20 | 1.871 | 1.854 | 1.863 | 1.704 | | | 10 | 1.192 | 1.168 | 1.180 | 1.021 | | | 5 | 0.667 | 0.643 | 0.655 | 0.496 | | | 2.5 | 0.466 | 0.455 | 0.461 | 0.302 | | | 1.25 | 0.310 | 0.289 | 0.300 | 0.141 | | | 0.625 | 0.256 | 0.246 | 0.251 | 0.092 | | | 0 | 0.160 | 0.158 | 0.159 | | |
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货期
3-5 working days