产品描述

This Human GLA ELISA Kit was designed for the quantitative measurement of Human GLA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

别名

AGAL_HUMAN ELISA Kit; Agalsidase alfa ELISA Kit; Alpha D galactosidase A ELISA Kit; Alpha D galactoside galactohydrolase 1 ELISA Kit; Alpha D galactoside galactohydrolase ELISA Kit; Alpha gal A ELISA Kit; Alpha galactosidase A ELISA Kit; Alpha-D-galactosidase A ELISA Kit; Alpha-D-galactoside galactohydrolase ELISA Kit; Alpha-galactosidase A ELISA Kit; GALA ELISA Kit; Galactosidase; alpha ELISA Kit; GLA ELISA Kit; GLA protein ELISA Kit; Melibiase ELISA Kit

缩写

GLA

Uniprot No

P06280

灵敏度

1.95 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human αGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		86
		Range %		81-92
1:200		Average %		92
		Range %		87-96
1:400		Average %		84
		Range %		81-86
1:800		Average %		102
		Range %		100-106

回收率

The recovery of human αGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		90-98
EDTA plasma (n=4)		88		84-93

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 500 2.420 2.451 2.436 2.272 250 2.065 2.107 2.086 1.922 125 1.663 1.705 1.684 1.520 62.5 1.165 1.183 1.174 1.010 31.2 0.735 0.788 0.762 0.598 15.6 0.482 0.499 0.491 0.327 7.8 0.328 0.341 0.335 0.171 0 0.162 0.166 0.164

货期

3-5 working days