产品描述
This Human CXCL3 ELISA Kit was designed for the quantitative measurement of Human CXCL3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.37 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL .
别名
C-X-C motif chemokine 3 ELISA Kit; C-X-C motif chemokine ligand 3 ELISA Kit; Chemokine (C X C motif) ligand 3 ELISA Kit; Chemokine (CXC motif) ligand 3 ELISA Kit; Cinc 2 ELISA Kit; CINC 2b ELISA Kit; Cinc2 ELISA Kit; CINC2b ELISA Kit; CXCL 3 ELISA Kit; Cxcl3 ELISA Kit; CXCL3_HUMAN ELISA Kit; Cytokine induced neutrophil chemoattractant 2 ELISA Kit; Dcip1 ELISA Kit; Dendritic cell inflammatory protein 1 ELISA Kit; Gm1960 ELISA Kit; GRO protein gamma ELISA Kit; GRO-gamma ELISA Kit; GRO-gamma(1-73) ELISA Kit; GRO-gamma(5-73) ELISA Kit; GRO3 ELISA Kit; GRO3 oncogene ELISA Kit; GROG ELISA Kit; Growth regulated protein gamma ELISA Kit; Growth-regulated protein gamma ELISA Kit; Macrophage inflammatory protein 2 beta precursor ELISA Kit; Macrophage inflammatory protein 2-beta ELISA Kit; Melanoma growth stimulatory activity gamma ELISA Kit; Member 3 ELISA Kit; MGSA gamma ELISA Kit; MIP 2b ELISA Kit; MIP2-beta ELISA Kit; MIP2B ELISA Kit; SCYB3 ELISA Kit; Small inducible cytokine subfamily B ELISA Kit
缩写
CXCL3
Uniprot No
P19876
灵敏度
2.34 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CXCL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 87-93 | |
| 1:2 | Average % | 100 | |
| Range % | 97-103 | |
| 1:4 | Average % | 95 | |
| Range % | 92-97 | |
| 1:8 | Average % | 99 | |
| Range % | 95-102 | |
回收率
| The recovery of human CXCL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 91-97 | |
| EDTA plasma (n=4) | 97 | 93-99 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 600 | 2.943 | 2.888 | 2.916 | 2.764 | | | 300 | 2.188 | 2.299 | 2.244 | 2.092 | | | 150 | 1.458 | 1.392 | 1.425 | 1.273 | | | 75 | 0.778 | 0.790 | 0.784 | 0.632 | | | 37.5 | 0.392 | 0.382 | 0.387 | 0.235 | | | 18.75 | 0.288 | 0.269 | 0.279 | 0.127 | | | 9.37 | 0.240 | 0.234 | 0.237 | 0.085 | | | 0 | 0.153 | 0.151 | 0.152 | | |
|
货期
3-5 working days