产品描述

This Human FABP4 ELISA Kit was designed for the quantitative measurement of Human FABP4 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

别名

3T3-L1 lipid-binding protein ELISA Kit; 422/aP2 ELISA Kit; A-FABP ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid binding protein ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; Adipocyte protein AP2 ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; AFABP ELISA Kit; ALBP ELISA Kit; ALBP/Ap2 ELISA Kit; aP2 ELISA Kit; Epididymis secretory protein Li 104 ELISA Kit; FABP ELISA Kit; FABP4 ELISA Kit; FABP4_HUMAN ELISA Kit; Fatty acid binding protein 4 adipocyte ELISA Kit; Fatty acid binding protein 4 ELISA Kit; Fatty acid binding protein adipocyte ELISA Kit; Fatty acid-binding protein 4 ELISA Kit; Fatty acid-binding protein ELISA Kit; HEL S 104 ELISA Kit; Lbpl ELISA Kit; Myelin P2 protein homolog ELISA Kit; P15 ELISA Kit; P2 adipocyte protein ELISA Kit; Protein 422 ELISA Kit

缩写

FABP4

Uniprot No

P15090

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FABP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:20		Average %		91
		Range %		86-98
1:40		Average %		97
		Range %		92-104
1:80		Average %		85
		Range %		81-88
1:160		Average %		89
		Range %		83-96

回收率

The recovery of human FABP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-97
EDTA plasma (n=4)		94		90-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average 4000 0.082 0.086 0.084 2000 0.145 0.134 0.140 1000 0.223 0.221 0.222 500 0.342 0.324 0.333 250 0.582 0.624 0.603 125 0.824 0.859 0.842 62.5 1.208 1.256 1.232 0 2.051 2.216 2.134

货期

3-5 working days