产品描述
This Mouse HDL-C ELISA Kit was designed for the quantitative measurement of Mouse HDL-C protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL .
缩写
HDL-C
灵敏度
1.95 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1000 | Average % | 88 | |
| Range % | 84-92 | |
| 1:2000 | Average % | 91 | |
| Range % | 85-97 | |
| 1:4000 | Average % | 99 | |
| Range % | 95-104 | |
| 1:8000 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of mouse HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-102 | |
| EDTA plasma (n=4) | 97 | 91-104 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.509 | 2.598 | 2.554 | 2.440 | | | 250 | 2.045 | 2.068 | 2.057 | 1.943 | | | 125 | 1.401 | 1.457 | 1.429 | 1.315 | | | 62.5 | 0.977 | 0.999 | 0.988 | 0.874 | | | 31.2 | 0.572 | 0.598 | 0.585 | 0.471 | | | 15.6 | 0.315 | 0.338 | 0.327 | 0.213 | | | 7.8 | 0.256 | 0.267 | 0.262 | 0.148 | | | 0 | 0.113 | 0.115 | 0.114 | | |
|
货期
3-5 working days