产品描述
This Rat F1+2 ELISA Kit was designed for the quantitative measurement of Rat F1+2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pmol/L-4000 pmol/L and the sensitivity is 15.6 pmol/L.
缩写
F1+2
灵敏度
15.6 pmol/L
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 89 | |
| Range % | 80-92 | |
| 1:2 | Average % | 100 | |
| Range % | 91-105 | |
| 1:4 | Average % | 102 | |
| Range % | 92-110 | |
| 1:8 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of rat F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-98 | |
| EDTA plasma (n=4) | 95 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pmol/L | OD1 | OD2 | Average | Corrected | | | 4000 | 2.739 | 2.726 | 2.733 | 2.622 | | | 2000 | 2.243 | 2.201 | 2.222 | 2.111 | | | 1000 | 1.447 | 1.441 | 1.444 | 1.333 | | | 500 | 0.802 | 0.739 | 0.771 | 0.660 | | | 250 | 0.427 | 0.414 | 0.421 | 0.310 | | | 125 | 0.269 | 0.252 | 0.261 | 0.150 | | | 62.5 | 0.178 | 0.166 | 0.172 | 0.061 | | | 0 | 0.113 | 0.109 | 0.111 | | |
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货期
3-5 working days