产品描述
This Human D-Dimer (D2D) ELISA Kit is suitable for qualitatively determining D2D concentrations in multiple biological fluids, including human serum and plasma in vitro. D-dimer is the final degradation product of cross-linked fibrin generated during plasma coagulation. Yumeng Yao etc. demonstrated that D-dimer expression is commonly elevated in patients infected with SARS-CoV-2. D-dimer levels correlate with disease severity and are a reliable prognostic marker for in-hospital mortality in patients admitted for COVID-19.
This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human D2D in the sample. Standards and samples are respectively added to the microplate wells pre-coated with anti-human D2D antibody. Biotin-labeled D2D antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. D2D in the sample or standard is captured by the immobilized antibody and then binds to the biotinylated D2D monoclonal antibody. And the biotin on the biotinylated D2D antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of TMB substrate. The addition of the stop solution into the wells immediately turns the blue solution into a yellow one. The concentration of D2D in the samples is directly proportional to the OD value (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency. See the manual for more information on validation.
缩写
D2D
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
87 |
|
| Range % |
80-95 |
|
| 1:2 |
Average % |
96 |
|
| Range % |
91-100 |
|
| 1:4 |
Average % |
102 |
|
| Range % |
92-110 |
|
| 1:8 |
Average % |
92 |
|
| Range % |
84-98 |
|
回收率
| The recovery of human D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
80-96 |
|
| EDTA plasma (n=4) |
95 |
90-105 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.695 |
2.498 |
2.597 |
2.457 |
|
| 500 |
2.289 |
2.138 |
2.214 |
2.074 |
|
| 250 |
1.544 |
1.486 |
1.515 |
1.375 |
|
| 125 |
0.813 |
0.783 |
0.798 |
0.658 |
|
| 62.5 |
0.415 |
0.401 |
0.408 |
0.268 |
|
| 31.2 |
0.279 |
0.274 |
0.277 |
0.137 |
|
| 15.6 |
0.217 |
0.211 |
0.214 |
0.074 |
|
| 0 |
0.141 |
0.138 |
0.140 |
|
|
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
