产品描述

This Human CYP2C9 ELISA Kit was designed for the quantitative measurement of Human CYP2C9 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

(R)-limonene 6-monooxygenase ELISA Kit; (S)-limonene 6-monooxygenase ELISA Kit; (S)-limonene 7-monooxygenase ELISA Kit; CP2C9_HUMAN ELISA Kit; CPC9 ELISA Kit; CYP2C ELISA Kit; CYP2C10 ELISA Kit; CYP2C9 ELISA Kit; CYPIIC9 ELISA Kit; cytochrome P-450 S-mephenytoin 4-hydroxylase ELISA Kit; Cytochrome P-450MP ELISA Kit; Cytochrome P450 2C9 ELISA Kit; Cytochrome P450 MP-4 ELISA Kit; Cytochrome P450 MP-8 ELISA Kit; Cytochrome P450 PB-1 ELISA Kit; Cytochrome P450; family 2; subfamily C; polypeptide 9 ELISA Kit; Cytochrome p4502C9 ELISA Kit; flavoprotein-linked monooxygenase ELISA Kit; MGC149605 ELISA Kit; MGC88320 ELISA Kit; microsomal monooxygenase ELISA Kit; OTTHUMP00000020135 ELISA Kit; P450 MP ELISA Kit; P450 PB 1 ELISA Kit; P450 PB1 ELISA Kit; P450IIC9 ELISA Kit; P450MP ELISA Kit; S mephenytoin 4 hydroxylase ELISA Kit; S-mephenytoin 4-hydroxylase ELISA Kit; xenobiotic monooxygenase ELISA Kit

缩写

CYP2C9

Uniprot No

P11712

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP2C9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		105
		Range %		94-109
1:2		Average %		97
		Range %		89-101
1:4		Average %		93
		Range %		83-98
1:8		Average %		107
		Range %		94-111

回收率

The recovery of human CYP2C9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		81-94
EDTA plasma (n=4)		105		97-109

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     1500 2.497 2.389 2.443 2.277     750 1.795 1.767 1.781 1.615     375 1.076 1.062 1.069 0.903     187.5 0.605 0.595 0.600 0.434     94 0.376 0.366 0.371 0.205     47 0.248 0.258 0.253 0.087     23.5 0.215 0.211 0.213 0.047     0 0.169 0.163 0.166

货期

3-5 working days