产品描述
The bovine haptoglobin (HP) ELISA Kit is engineered for accurate measurement of bovine haptoglobin levels from samples including serum or plasma. It uses the cmpetitive-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the bovine haptoglobin content in the sample. The color intensity is negatively correlated with haptoglobin content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
Haptoglobin is an acute-phase protein that is synthesized mainly in the liver and lungs and is crucial for the elimination of free hemoglobin and the neutralization of oxidative damage. It has powerful regulatory activity on lymphocytes. Additionally, haptoglobin exhibits immunoregulatory properties, participates in angiogenesis and inhibition of nitric oxide, stimulates tissue repair, etc.
别名
HPHaptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
缩写
HP
Uniprot No
Q2TBU0
灵敏度
7.81 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1000 |
Average % |
84 |
|
| Range % |
80-88 |
|
| 1:2000 |
Average % |
90 |
|
| Range % |
85-94 |
|
| 1:4000 |
Average % |
103 |
|
| Range % |
99-112 |
|
| 1:8000 |
Average % |
98 |
|
| Range % |
94-102 |
|
回收率
| The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-99 |
|
| EDTA plasma (n=4) |
92 |
88-96 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
|
|
| 2000 |
0.243 |
0.252 |
0.248 |
|
|
| 500 |
0.412 |
0.436 |
0.424 |
|
|
| 120 |
0.793 |
0.754 |
0.774 |
|
|
| 31.25 |
1.214 |
1.239 |
1.227 |
|
|
| 7.81 |
1.795 |
1.724 |
1.760 |
|
|
| 0 |
2.405 |
2.359 |
2.382 |
|
|
|
货期
3-5 working days
已关闭
固定模块
收藏
易享客服
