产品描述
The human HO-1 ELISA Kit is engineered for accurate measurement of human HO-1 levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the HO-1 content in the sample. The color intensity is positively correlated with HO-1 content in the sample. The HO-1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
HO-1, also called HMOX1, is the inducible isoform of heme oxygenase (HO) that is expressed in response to various stimuli to degrade heme leading to the generation of biliverdin, carbon monoxide, and release of ferrous iron. In essence, HO-1 exerts antioxidant, anti-apoptotic, and cytoprotective functions through its metabolic products as well as scavenging toxic intracellular heme. It also plays an important role in the prevention of vascular inflammation. HO-1 expression has been detected in a variety of cancers, including bladder, breast, and colorectal cancers.
别名
32 kD ELISA Kit; bK286B10 ELISA Kit; D8Wsu38e ELISA Kit; heat shock protein 32 kD ELISA Kit; heat shock protein 32kD ELISA Kit; Heat shock protein ELISA Kit; Heme oxygenase (decycling) 1 ELISA Kit; Heme oxygenase 1 ELISA Kit; Hemox ELISA Kit; HMOX 1 ELISA Kit; Hmox ELISA Kit; Hmox1 ELISA Kit; HMOX1_HUMAN ELISA Kit; HO 1 ELISA Kit; HO ELISA Kit; HO-1 ELISA Kit; HO1 ELISA Kit; Hsp32 ELISA Kit
缩写
HMOX1
Uniprot No
P09601
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-94 | |
| 1:2 | Average % | 95 | |
| Range % | 89-99 | |
| 1:4 | Average % | 98 | |
| Range % | 92-105 | |
| 1:8 | Average % | 94 | |
| Range % | 88-99 | |
回收率
| The recovery of human HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-92 | |
| EDTA plasma (n=4) | 98 | 90-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.449 | 2.284 | 2.367 | 2.263 | | | 500 | 1.967 | 1.864 | 1.916 | 1.812 | | | 250 | 1.277 | 1.233 | 1.255 | 1.151 | | | 125 | 0.701 | 0.674 | 0.688 | 0.584 | | | 62.5 | 0.430 | 0.421 | 0.426 | 0.322 | | | 31.2 | 0.258 | 0.253 | 0.256 | 0.152 | | | 15.6 | 0.187 | 0.184 | 0.186 | 0.082 | | | 0 | 0.104 | 0.103 | 0.104 | | |
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货期
3-5 working days
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