产品描述
The Fish Aromatase (ARO) ELISA Kit is suitable for the quantitative detection of ARO in fish samples, including serum, plasma, and tissue homogenates. It has been validated with excellent sensitivity, high specificity, premium precision, high recovery, and lot-to-lot consistency. See the product instructions for more details.
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with goat-anti-rabbit ARO antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for ARO and HRP-conjugated ARO. The competitive inhibition reaction is launched between with HRP-labeled ARO and ARO in the samples or standards with the anti-fish ARO antibody. The substrate solution is added to the wells and the color develops in opposite to the amount of ARO in the sample. The color development is stopped and the intensity of the color is measured.
ARO is the enzyme responsible for the aromatization of androgens to estrogen in many tissues, such as gonads, adipocytes, and the brain. It is important for sexual development. ARO helps make the estrogen that is conducive to fuel the growth of hormone-receptor-positive breast cancer. It may play a role in sex-determination. High levels of ARO was expressed at temperatures that yield female progeny in temperature-dependent sex determinant species. ARO also exerts a neuroprotective role. Many factors, including age, obesity, insulin, alcohol, and smoking, can enhance the activity of ARO.
别名
cyp19a1 ELISA kit; cyp19Aromatase ELISA kit; EC 1.14.14.14 ELISA kit; CYPXIX ELISA kit; Cytochrome P-450AROM ELISA kit; Cytochrome P450 19A1 ELISA kit; Estrogen synthase ELISA kit
缩写
CYP19A1
Uniprot No
Q92087
灵敏度
25 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish ARO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
89-104 |
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| 1:2 |
Average % |
95 |
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| Range % |
86-100 |
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| 1:4 |
Average % |
95 |
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| Range % |
87-106 |
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| 1:8 |
Average % |
89 |
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| Range % |
86-99 |
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回收率
| The recovery of fish ARO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
89-103 |
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| EDTA plasma (n=4) |
94 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
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| 1000 |
0.154 |
0.156 |
0.155 |
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| 500 |
0.359 |
0.355 |
0.357 |
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| 230 |
0.573 |
0.564 |
0.569 |
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| 75 |
0.877 |
0.862 |
0.870 |
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| 25 |
1.561 |
1.583 |
1.572 |
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| 0 |
2.342 |
2.369 |
2.356 |
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货期
3-5 working days
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