产品描述

The human FGF23 ELISA Kit allows for the in vitro quantitative determination of FGF23 concentrations in serum, cell culture supernates, urine, or tissue homogenates. This kit exclusively recognizes human FGF23. FGF23 is a bone-derived hormone that inhibits phosphate reabsorption and vitamin D hormone synthesis in the kidney. FGF23 signals through α-Klotho (KLA) and FGF receptor (FGFR) leading to kinase activation, modulation of phosphate homeostasis, vitamin D levels, and calcium metabolism. Increased FGF23 abundance is responsible for several hereditary and acquired hypophosphatemic rickets disorders or tumor-induced osteomalacia. Deficiency of FGF23 results in elevated phosphate levels, such as in familiar tumor calcinosis.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. FGF23 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled FGF23 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with FGF23 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.

别名

ADHR ELISA Kit; FGF-23 ELISA Kit; Fgf23 ELISA Kit; FGF23_HUMAN ELISA Kit; FGFN ELISA Kit; Fibroblast growth factor 23 ELISA Kit; Fibroblast growth factor 23 C-terminal peptide ELISA Kit; Fibroblast growth factor 23 precursor ELISA Kit; HPDR2 ELISA Kit; HYPF ELISA Kit; Phosphatonin ELISA Kit; PHPTC ELISA Kit; Tumor derived hypophosphatemia inducing factor ELISA Kit; Tumor-derived hypophosphatemia-inducing factor ELISA Kit

缩写

FGF23

Uniprot No

Q9GZV9

灵敏度

0.78 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		99
		Range %		80-115
1:2		Average %		100
		Range %		91-110
1:4		Average %		97
		Range %		92-115
1:8		Average %		93
		Range %		86-100

回收率

The recovery of human FGF-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 200 2.958 2.986 2.972 2.783 100 2.461 2.475 2.468 2.279 50 1.852 1.889 1.871 1.682 25 1.234 1.286 1.260 1.071 12.5 0.753 0.786 0.770 0.581 6.25 0.531 0.556 0.544 0.355 3.12 0.396 0.416 0.406 0.217 0 0.192 0.186 0.189

货期

3-5 working days