产品描述
This Human ENHO ELISA Kit was designed for the quantitative measurement of Human ENHO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
别名
Adropin ELISA Kit; C9orf165 ELISA Kit; Energy homeostasis associated protein ELISA Kit; Energy homeostasis-associated protein ELISA Kit; ENHO ELISA Kit; ENHO_HUMAN ELISA Kit; PRO830 ELISA Kit; UNQ470 ELISA Kit
缩写
ENHO
Uniprot No
Q6UWT2
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 94 | |
| Range % | 88-99 | |
| 1:200 | Average % | 90 | |
| Range % | 84-95 | |
| 1:400 | Average % | 98 | |
| Range % | 91-99 | |
| 1:800 | Average % | 95 | |
| Range % | 90-100 | |
回收率
| The recovery of human ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 87 | 80-94 | |
| EDTA plasma (n=4) | 100 | 94-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.074 | 2.099 | 2.087 | 1.923 | | | 50 | 1.798 | 1.765 | 1.782 | 1.618 | | | 25 | 1.397 | 1.384 | 1.391 | 1.227 | | | 12.5 | 0.982 | 0.943 | 0.963 | 0.799 | | | 6.25 | 0.583 | 0.596 | 0.590 | 0.426 | | | 3.12 | 0.371 | 0.391 | 0.381 | 0.217 | | | 1.56 | 0.296 | 0.281 | 0.289 | 0.125 | | | 0 | 0.162 | 0.166 | 0.164 | | |
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货期
3-5 working days