产品描述
This Rat IFNA1 ELISA Kit was designed for the quantitative measurement of Rat IFNA1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit
缩写
IFN-α
Uniprot No
P05011
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-91 |
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| 1:2 |
Average % |
92 |
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| Range % |
85-97 |
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| 1:4 |
Average % |
99 |
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| Range % |
94-104 |
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| 1:8 |
Average % |
103 |
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| Range % |
99-108 |
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回收率
| The recovery of rat IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-96 |
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| EDTA plasma (n=4) |
96 |
90-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.087 |
2.099 |
2.093 |
1.916 |
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| 1000 |
1.595 |
1.574 |
1.585 |
1.408 |
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| 500 |
1.087 |
1.096 |
1.092 |
0.915 |
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| 250 |
0.672 |
0.695 |
0.684 |
0.507 |
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| 125 |
0.491 |
0.457 |
0.474 |
0.297 |
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| 62.5 |
0.376 |
0.351 |
0.364 |
0.187 |
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| 31.25 |
0.287 |
0.299 |
0.293 |
0.116 |
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| 0 |
0.175 |
0.178 |
0.177 |
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货期
3-5 working days