产品描述
This Human CYFRA21-1 ELISA Kit was designed for the quantitative measurement of Human CYFRA21-1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
缩写
CYFRA21-1
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYFRA21-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-90 | |
| 1:2 | Average % | 98 | |
| Range % | 91-105 | |
| 1:4 | Average % | 99 | |
| Range % | 94-110 | |
| 1:8 | Average % | 92 | |
| Range % | 86-99 | |
回收率
| The recovery of human CYFRA21-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 80-98 | |
| EDTA plasma (n=4) | 97 | 90-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.541 | 2.385 | 2.463 | 2.305 | | | 10 | 2.229 | 2.082 | 2.156 | 1.998 | | | 5 | 1.607 | 1.545 | 1.576 | 1.418 | | | 2.5 | 0.866 | 0.842 | 0.854 | 0.696 | | | 1.25 | 0.463 | 0.439 | 0.451 | 0.293 | | | 0.625 | 0.337 | 0.324 | 0.331 | 0.173 | | | 0.312 | 0.254 | 0.246 | 0.250 | 0.092 | | | 0 | 0.160 | 0.156 | 0.158 | | |
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货期
3-5 working days