产品描述
The rat ALT ELISA Kit is engineered for accurate measurement of rat ALT levels from samples including serum, plasma, cell culture supernates, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the ALT content in the sample. The color intensity is positively correlated with ALT content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
ALT, also called GPT, catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate in the alanine cycle to form pyruvate and glutamate. The ALT enzyme is primarily found in the liver. ALT concentration is increased with liver damage and is used to screen for or monitor liver disease. An ALT blood test is often included in a liver panel and comprehensive metabolic panel and is applied to help assess liver health. High levels of ALT in your blood may indicate that you have damage to your liver or a liver condition.
别名
Gpt ELISA Kit; Aat1 ELISA Kit; Gpt1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; ALT1 ELISA Kit; EC 2.6.1.2 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; GPT 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit
缩写
GPT
Uniprot No
P25409
灵敏度
0.78 mIU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 80-92 | |
| 1:2 | Average % | 98 | |
| Range % | 91-105 | |
| 1:4 | Average % | 100 | |
| Range % | 92-110 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of rat ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 96 | 90-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | mIU/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.814 | 2.911 | 2.863 | 2.778 | | | 100 | 2.466 | 2.355 | 2.411 | 2.326 | | | 50 | 1.659 | 1.564 | 1.612 | 1.527 | | | 25 | 0.853 | 0.812 | 0.833 | 0.748 | | | 12.5 | 0.469 | 0.442 | 0.456 | 0.371 | | | 6.25 | 0.243 | 0.226 | 0.235 | 0.150 | | | 3.12 | 0.149 | 0.141 | 0.145 | 0.060 | | | 0 | 0.088 | 0.082 | 0.085 | | |
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货期
3-5 working days
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