产品描述

This Human CYP7A1 ELISA Kit was designed for the quantitative measurement of Human CYP7A1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

别名

Cholesterol 7 alpha hydroxylase ELISA Kit; Cholesterol 7 alpha monooxygenase ELISA Kit; Cholesterol 7-alpha-hydroxylase ELISA Kit; Cholesterol 7-alpha-monooxygenase ELISA Kit; CP7A ELISA Kit; CP7A1_HUMAN ELISA Kit; CYP 7 ELISA Kit; CYP7 ELISA Kit; CYP7A1 ELISA Kit; CYPVII ELISA Kit; Cytochrome P450 7A1 ELISA Kit; Cytochrome P450; family 7; subfamily A; polypeptide 1 ELISA Kit; Cytochrome P450; subfamily VIIA (cholesterol 7 alpha monooxygenase); ELISA Kit; Cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1 ELISA Kit; MGC126826 ELISA Kit; MGC138389 ELISA Kit

缩写

CYP7A1

Uniprot No

P22680

灵敏度

4.7 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CYP7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-90
1:2		Average %		94
		Range %		90-100
1:4		Average %		92
		Range %		85-97
1:8		Average %		92
		Range %		87-98

回收率

The recovery of human CYP7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		87-98
EDTA plasma (n=4)		91		85-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1200 2.606 2.520 2.563 2.420 600 1.912 1.769 1.841 1.698 300 1.070 1.004 1.037 0.894 150 0.686 0.639 0.663 0.520 75 0.408 0.394 0.401 0.258 37.5 0.314 0.309 0.312 0.169 18.75 0.216 0.199 0.208 0.065 0 0.149 0.137 0.143

货期

3-5 working days