产品描述

This Human HMGB1 ELISA Kit was designed for the quantitative measurement of Human HMGB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

别名

Amphoterin ELISA Kit; Chromosomal protein; nonhistone; HMG1 ELISA Kit; DKFZp686A04236 ELISA Kit; High mobility group 1 ELISA Kit; High mobility group box 1 ELISA Kit; High mobility group protein 1 ELISA Kit; High mobility group protein B1 ELISA Kit; high-mobility group (nonhistone chromosomal) protein 1 ELISA Kit; HMG-1 ELISA Kit; HMG1 ELISA Kit; HMG3 ELISA Kit; HMGB 1 ELISA Kit; HMGB1 ELISA Kit; HMGB1_HUMAN ELISA Kit; NONHISTONE CHROMOSOMAL PROTEIN HMG1 ELISA Kit; SBP 1 ELISA Kit; Sulfoglucuronyl carbohydrate binding protein ELISA Kit

缩写

HMGB1

Uniprot No

P09429

灵敏度

19.5 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

90

Range %

84-99

1:2

Average %

88

Range %

82-94

1:4

Average %

95

Range %

89-102

1:8

Average %

94

Range %

88-100

回收率

The recovery of human HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type			Average % Recovery				Range
Serum (n=5)			98				90-102
EDTA plasma (n=4)			91				85-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 5000 1.912 1.831 1.872 1.780 2500 1.454 1.369 1.412 1.320 1250 0.839 0.789 0.814 0.722 625 0.456 0.487 0.472 0.380 312 0.283 0.297 0.290 0.198 156 0.199 0.202 0.201 0.109 78 0.103 0.108 0.106 0.014 0 0.089 0.094 0.092

货期

3-5 working days