产品描述
This Rat Glp2 ELISA Kit was designed for the quantitative measurement of Rat Glp2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
N/A
缩写
Glp2
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 89 | | |
| Range % | 82-95 | | |
| 1:2 | Average % | 98 | | |
| Range % | 93-103 | | |
| 1:4 | Average % | 97 | | |
| Range % | 90-104 | | |
| 1:8 | Average % | 86 | | |
| Range % | 82-91 | | |
回收率
| The recovery of rat GLP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 87-99 | | |
| EDTA plasma (n=4) | 99 | 96-104 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.057 | 2.052 | 2.055 | 1.912 | | | | 500 | 1.603 | 1.633 | 1.618 | 1.475 | | | | 250 | 1.212 | 1.228 | 1.220 | 1.077 | | | | 125 | 0.846 | 0.911 | 0.879 | 0.736 | | | | 62.5 | 0.559 | 0.543 | 0.551 | 0.408 | | | | 31.2 | 0.332 | 0.351 | 0.342 | 0.199 | | | | 15.6 | 0.252 | 0.265 | 0.259 | 0.116 | | | | 0 | 0.142 | 0.144 | 0.143 | | | |
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货期
3-5 working days