产品描述

This Human EPHA2 ELISA Kit was designed for the quantitative measurement of Human EPHA2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

ARCC2 ELISA Kit; AW545284 ELISA Kit; CTPA ELISA Kit; CTPP1 ELISA Kit; CTRCT6 ELISA Kit; EC 2.7.10.1 ELISA Kit; Eck ELISA Kit; Eph receptor A2 ELISA Kit; EPHA2 ELISA Kit; EPHA2_HUMAN ELISA Kit; Ephrin receptor ELISA Kit; Ephrin receptor EphA2 ELISA Kit; Ephrin type A receptor 2 ELISA Kit; Ephrin type-A receptor 2 ELISA Kit; Epithelial cell kinase ELISA Kit; Epithelial cell receptor protein tyrosine kinase ELISA Kit; Myk 2 ELISA Kit; Myk2 ELISA Kit; Sek 2 ELISA Kit; Sek2 ELISA Kit; Soluble EPHA2 variant 1 ELISA Kit; Tyrosine protein kinase receptor ECK ELISA Kit; Tyrosine-protein kinase receptor ECK ELISA Kit; Tyrosine-protein kinase receptor MPK-5 ELISA Kit; Tyrosine-protein kinase receptor SEK-2 ELISA Kit

缩写

EPHA2

Uniprot No

P29317

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human EPHA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		85-97
1:2		Average %		100
		Range %		95-105
1:4		Average %		94
		Range %		89-99
1:8		Average %		95
		Range %		90-100

回收率

The recovery of human EPHA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		89-101
EDTA plasma (n=4)		92		85-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 10 2.464 2.575 2.520 2.401 5 1.768 1.889 1.829 1.710 2.5 1.236 1.265 1.251 1.132 1.25 0.712 0.766 0.739 0.620 0.625 0.407 0.435 0.421 0.302 0.312 0.254 0.264 0.259 0.140 0.156 0.228 0.245 0.237 0.118 0 0.116 0.121 0.119

货期

3-5 working days