产品描述

This Human ENO1 ELISA Kit was designed for the quantitative measurement of Human ENO1 protein in serum, tissue homogenates, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

别名

2 phospho D glycerate hydro lyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Alpha enolase ELISA Kit; Alpha enolase like 1 ELISA Kit; Alpha-enolase ELISA Kit; C myc promoter binding protein ELISA Kit; C-myc promoter-binding protein ELISA Kit; EC 4.2.1.11 ELISA Kit; eno1 ELISA Kit; ENO1L1 ELISA Kit; ENOA_HUMAN ELISA Kit; Enolase 1 (alpha) ELISA Kit; Enolase 1 (alpha) like 1 ELISA Kit; Enolase 1 ELISA Kit; Enolase alpha ELISA Kit; MBP 1 ELISA Kit; MBP-1 ELISA Kit; MBP1 ELISA Kit; MBPB1 ELISA Kit; MPB 1 ELISA Kit; MPB-1 ELISA Kit; MPB1 ELISA Kit; MYC promoter binding protein 1 ELISA Kit; NNE ELISA Kit; Non neural enolase ELISA Kit; Non-neural enolase ELISA Kit; Phosphopyruvate hydratase ELISA Kit; Plasminogen binding protein ELISA Kit; Plasminogen-binding protein ELISA Kit; PPH ELISA Kit; Tau crystallin ELISA Kit

缩写

ENO1

Uniprot No

P06733

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ENO1/ENO1L1/MBPB1/MPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-97
1:2		Average %		92
		Range %		88-96
1:4		Average %		94
		Range %		89-98
1:8		Average %		100
		Range %		96-105

回收率

The recovery of human ENO1/ENO1L1/MBPB1/MPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-100
EDTA plasma (n=4)		96		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.321 2.319 2.320 2.225 10 1.982 1.967 1.975 1.880 5 1.470 1.576 1.523 1.428 2.5 1.003 0.995 0.999 0.904 1.25 0.638 0.652 0.645 0.550 0.625 0.380 0.391 0.386 0.291 0.312 0.293 0.299 0.296 0.201 0 0.093 0.097 0.095

货期

3-5 working days