产品描述

This Human HIST1H3A ELISA Kit was designed for the quantitative measurement of Human HIST1H3A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

H3 histone family member E pseudogene ELISA Kit; H3 histone family; member A ELISA Kit; H3/A ELISA Kit; H31_HUMAN ELISA Kit; H3F3 ELISA Kit; H3FA ELISA Kit; Hist1h3a ELISA Kit; HIST1H3B ELISA Kit; HIST1H3C ELISA Kit; HIST1H3D ELISA Kit; HIST1H3E ELISA Kit; HIST1H3F ELISA Kit; HIST1H3G ELISA Kit; HIST1H3H ELISA Kit; HIST1H3I ELISA Kit; HIST1H3J ELISA Kit; HIST3H3 ELISA Kit; histone 1; H3a ELISA Kit; Histone cluster 1; H3a ELISA Kit; Histone H3 3 pseudogene ELISA Kit; Histone H3.1 ELISA Kit; Histone H3/a ELISA Kit; Histone H3/b ELISA Kit; Histone H3/c ELISA Kit; Histone H3/d ELISA Kit; Histone H3/f ELISA Kit; Histone H3/h ELISA Kit; Histone H3/i ELISA Kit; Histone H3/j ELISA Kit; Histone H3/k ELISA Kit; Histone H3/l ELISA Kit

缩写

HIST1H3A

Uniprot No

P68431

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		84
		Range %		80-93
1:2		Average %		88
		Range %		80-95
1:4		Average %		90
		Range %		85-98
1:8		Average %		95
		Range %		90-102

回收率

The recovery of human HIST1H3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		90-105
EDTA plasma (n=4)		92		87-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1500 2.157 2.134 2.146 2.004   750 1.623 1.562 1.593 1.451   375 1.158 1.082 1.120 0.978   187.5 0.746 0.697 0.722 0.580   94 0.471 0.451 0.461 0.319   47 0.348 0.321 0.335 0.193   23.5 0.253 0.249 0.251 0.109   0 0.148 0.136 0.142

货期

3-5 working days