产品描述
This Mouse HMGB1 ELISA Kit was designed for the quantitative measurement of Mouse HMGB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Hmgb1 ELISA Kit; Hmg-1 ELISA Kit; Hmg1High mobility group protein B1 ELISA Kit; High mobility group protein 1 ELISA Kit; HMG-1 ELISA Kit
缩写
HMGB1
Uniprot No
P63158
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Epigenetics and Nuclear Signaling
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 92 | |
| Range % | 80-98 | |
| 1:200 | Average % | 85 | |
| Range % | 81-96 | |
| 1:400 | Average % | 97 | |
| Range % | 90-104 | |
| 1:800 | Average % | 95 | |
| Range % | 82-98 | |
回收率
| The recovery of mouse HMGB-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 97 | 95-100 | |
| EDTA plasma (n=4) | 95 | 90-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.756 | 2.712 | 2.734 | 2.565 | | | 500 | 2.223 | 2.251 | 2.237 | 2.068 | | | 250 | 1.547 | 1.572 | 1.560 | 1.391 | | | 125 | 1.020 | 0.986 | 1.003 | 0.834 | | | 62.5 | 0.598 | 0.621 | 0.610 | 0.441 | | | 31.2 | 0.452 | 0.432 | 0.442 | 0.273 | | | 15.6 | 0.287 | 0.275 | 0.281 | 0.112 | | | 0 | 0.173 | 0.164 | 0.169 | | |
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货期
3-5 working days