产品描述
This Human DSG1 ELISA Kit was designed for the quantitative measurement of Human DSG1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.
别名
DSG1; CDHF4; Desmoglein-1; Cadherin family member 4; Desmosomal glycoprotein 1; DG1; DGI; Pemphigus foliaceus antigen
缩写
DSG1
Uniprot No
Q02413
灵敏度
5.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Dsg-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 92-102 | |
| 1:2 | Average % | 94 | |
| Range % | 86-98 | |
| 1:4 | Average % | 93 | |
| Range % | 89-97 | |
| 1:8 | Average % | 99 | |
| Range % | 95-103 | |
回收率
| The recovery of human Dsg-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 87-96 | |
| EDTA plasma (n=4) | 87 | 82-92 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1500 | 2.308 | 2.512 | 2.410 | 2.285 | | | 750 | 1.342 | 1.445 | 1.394 | 1.269 | | | 375 | 0.779 | 0.757 | 0.768 | 0.643 | | | 187.5 | 0.443 | 0.457 | 0.450 | 0.325 | | | 94 | 0.312 | 0.303 | 0.308 | 0.183 | | | 47 | 0.231 | 0.234 | 0.233 | 0.108 | | | 23.5 | 0.198 | 0.192 | 0.195 | 0.070 | | | 0 | 0.124 | 0.125 | 0.125 | | |
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货期
3-5 working days