产品描述
This Pig IGF2 ELISA Kit was designed for the quantitative measurement of Pig IGF2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
别名
IGF2Insulin-like growth factor II ELISA Kit; IGF-II) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
缩写
IGF2
Uniprot No
P23695
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:20 | Average % | 89 | | |
| Range % | 83-97 | | |
| 1:40 | Average % | 94 | | |
| Range % | 90-103 | | |
| 1:80 | Average % | 92 | | |
| Range % | 86-100 | | |
| 1:160 | Average % | 93 | | |
| Range % | 87-101 | | |
回收率
| The recovery of pig IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 97 | 91-105 | | |
| EDTA plasma (n=4) | 95 | 89-105 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 40 | 2.754 | 2.714 | 2.734 | 2.565 | | | | 20 | 2.144 | 2.126 | 2.135 | 1.966 | | | | 10 | 1.439 | 1.457 | 1.448 | 1.279 | | | | 5 | 0.925 | 0.932 | 0.929 | 0.760 | | | | 2.5 | 0.525 | 0.533 | 0.529 | 0.360 | | | | 1.25 | 0.381 | 0.364 | 0.373 | 0.204 | | | | 0.625 | 0.255 | 0.249 | 0.252 | 0.083 | | | | 0 | 0.170 | 0.168 | 0.169 | | | |
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货期
3-5 working days