产品描述
This Dog IFN-A1/2 ELISA Kit was designed for the quantitative measurement of Dog IFN-A1/2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.
别名
Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
缩写
IFN-α1/2
Uniprot No
P81255
灵敏度
3.12 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 86-99 | |
| 1:2 | Average % | 100 | |
| Range % | 94-109 | |
| 1:4 | Average % | 92 | |
| Range % | 89-96 | |
| 1:8 | Average % | 87 | |
| Range % | 83-96 | |
回收率
| The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-96 | |
| EDTA plasma (n=4) | 94 | 88-99 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 2.815 | 2.824 | 2.820 | 2.726 | | | 400 | 2.417 | 2.425 | 2.421 | 2.327 | | | 200 | 1.834 | 1.863 | 1.849 | 1.755 | | | 100 | 1.042 | 1.112 | 1.077 | 0.983 | | | 50 | 0.518 | 0.563 | 0.541 | 0.447 | | | 25 | 0.248 | 0.269 | 0.259 | 0.165 | | | 12.5 | 0.126 | 0.131 | 0.129 | 0.035 | | | 0 | 0.091 | 0.096 | 0.094 | | |
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货期
3-5 working days