产品描述

The human hypoxia-inducible factor 2 (HIF-2) ELISA Kit is used to quantitatively measure human HIF-2 levels in serum, plasma, cell lysates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of HIF-2 in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.

HIF-2, also known as EPAS1, is essential in maintaining proper micronutrient balance, the inflammatory response, and the regenerative and proliferative capacity of the intestine after an acute injury. It also plays important role in digestive system cancers and promotes the transcription of genes involved in cell proliferation, angiogenesis and apoptosis, metabolism, as well as metastasis and resistance to chemotherapy. However, chronic activation of HIF-2 results in an enhanced proinflammatory response, intestinal injury, and colorectal cancer.

别名

Basic helix loop helix PAS protein MOP2 ELISA Kit; Basic-helix-loop-helix-PAS protein MOP2 ELISA Kit; bHLHe73 ELISA Kit; Class E basic helix-loop-helix protein 73 ELISA Kit; ECYT4 ELISA Kit; Endothelial PAS domain containing protein 1 ELISA Kit; Endothelial pas domain protein 1 ELISA Kit; Endothelial PAS domain-containing protein 1 ELISA Kit; EPAS 1 ELISA Kit; EPAS-1 ELISA Kit; EPAS1 ELISA Kit; EPAS1_HUMAN ELISA Kit; HIF 1 alpha like factor ELISA Kit; HIF 2 alpha ELISA Kit; HIF-1-alpha-like factor ELISA Kit; HIF-2-alpha ELISA Kit; HIF2-alpha ELISA Kit; HIF2A ELISA Kit; HLF ELISA Kit; Hypoxia inducible factor 2 alpha ELISA Kit; Hypoxia inducible factor 2 alpha subunit ELISA Kit; Hypoxia-inducible factor 2-alpha ELISA Kit; Member of PAS protein 2 ELISA Kit; Member of pas superfamily 2 ELISA Kit; MOP 2 ELISA Kit; MOP2 ELISA Kit; PAS domain-containing protein 2 ELISA Kit; PASD2 ELISA Kit

缩写

EPAS1

Uniprot No

Q99814

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cardiovascular

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human HIF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		82-93
1:2		Average %		98
		Range %		94-100
1:4		Average %		97
		Range %		90-104
1:8		Average %		85
		Range %		82-93

回收率

The recovery of human HIF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-95
EDTA plasma (n=4)		101		96-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 4000 2.760 2.740 2.750 2.605 2000 2.184 2.350 2.267 2.122 1000 1.439 1.520 1.480 1.335 500 0.712 0.730 0.721 0.576 250 0.445 0.431 0.438 0.293 125 0.352 0.342 0.347 0.202 62.5 0.251 0.265 0.258 0.113 0 0.144 0.145 0.145

货期

3-5 working days